Universal closed-tube barcoding for monitoring the shark and ray trade in megadiverse conservation hotspots

Universal closed-tube barcoding for monitoring the shark and ray trade in megadiverse conservation hotspots

Blog Article

Summary: Trade restrictions for endangered elasmobranch species exist to disincentivise their exploitation and curb their declines.However, trade monitoring is challenging due to product variety and the complexity of Advanced Wound Care - Impregnated Dressings import/export routes.We investigate the use of a portable, universal, DNA-based tool which would greatly facilitate in-situ monitoring.We collected shark and ray samples across the Island of Java, Indonesia, and selected 28 commonly encountered species (including 22 CITES-listed species) to test a recently developed real-time PCR single-assay originally developed for Ski de fond - Enfant - Fixations - Skate screening bony fish.In the absence of a bespoke elasmobranch identification online platform in the original FASTFISH-ID model, we employed a deep learning algorithm to recognize species based on DNA melt-curve signatures.

By combining visual and machine-learning assignment methods, we distinguished 25/28 species, 20 of which were CITES-listed.With further refinement, this method can improve monitoring of the elasmobranch trade worldwide, without a lab or species-specific assays.